PPAR gamma Transcription Factor Assay Kit (ab133101) (2024)

Overview

• Product name

  PPAR gamma Transcription Factor Assay Kit

• Detection method

  Colorimetric

• Sample type

  Adherent cells, Suspension cells, Nuclear Extracts

• Assay type

  Semi-quantitative

• Species reactivity

  Reacts with: Mouse, Rat, Human
  Predicted to work with: Mammals

• Product overview

  PPAR gamma Transcription Factor Assay kit ab133101 is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

  
  

  A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA)sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96 well plate. PPARs contained in a nuclear extract bind specifically to the PPRE. PPAR gamma is detected by addition of specific primary antibody directed against PPAR gamma. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR gamma will not crossreact with PPAR delta or PPAR alpha.

• Notes

  Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

  PPAR gamma is primarily expressed in adipose tissue and to a lesser extent in the colon, immune system and the retina. PPAR gamma was first identified as regulator of adipogenesis, but also plays an important role in cellular differentiation, insulin sensitization , atherosclerosis and cancer.

• Platform

  Microplate reader

Properties

• Storage instructions

  Please refer to protocols.

• Components	96 tests
  96-Well Plate Cover	1 unit
  Polysorbate 20	1 vial
  Transcription Factor Antibody Binding Buffer (10X)	1 x 3ml
  Transcription Factor Binding Assay Buffer (4X)	1 x 3ml
  Transcription Factor Developing Solution	1 x 12ml
  Transcription Factor Goat Anti-Rabbit HRP Conjugate	1 x 100µl
  Transcription Factor PPAR 96-Well Strip Plate	1 unit
  Transcription Factor PPAR Competitor dsDNA	1 vial
  Transcription Factor PPAR gamma Positive Control	1 vial
  Transcription Factor PPAR gamma Primary Antibody	1 vial
  Transcription Factor Reagent A	1 x 120µl
  Transcription Factor Stop Solution	1 x 12ml
  Wash Buffer Concentrate (400X)	1 x 5ml

• Research areas

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Neuroscience
  • Neurology process
  • Metabolism

  • Stem Cells
  • Mesenchymal Stem Cells
  • Adipogenesis

  • Cardiovascular
  • Atherosclerosis
  • Diabetes associated

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Fatty Acid Assays

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Lipid Metabolism Kits
  • Obesity

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Metabolism
  • Types of disease
  • Diabetes

  • Metabolism
  • Types of disease
  • Obesity

  • Metabolism
  • Types of disease
  • Heart disease

  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Transcription factor assay kits

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

• Function

  Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.

• Tissue specificity

  Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

• Involvement in disease

  Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
  Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
  Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.

• Sequence similarities

  Belongs to the nuclear hormone receptor family. NR1 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.

• Cellular localization

  Nucleus.

• Information by UniProt

• Alternative names

  • CIMT1
  • GLM1
  • NR1C3

  see all

• Database links

  • Entrez Gene: 5468 Human
  • Entrez Gene: 19016 Mouse
  • Entrez Gene: 25664 Rat
  • Omim: 601487 Human
  • SwissProt: P37231 Human
  • SwissProt: P37238 Mouse
  • SwissProt: O88275 Rat
  • Unigene: 162646 Human

  see all

• Functional Assay: ab133101 PPAR gamma Transcription Factor Assay Kit

  3T3-L1 cells were differentiated to adipocytes with 1 uM dexamethasone (ab120743), 1 ug x mL^-1 insulin (ab123768) and 0.5 mM IBMX (ab120840). From the third day, the cells were grown in normal medium with the addition of only 1 ug x mL^-1 insulin. From day six, the cells were cultured in normal medium for an additional two days. 40 uL of nuclear extracts were tested in duplicates (+/- SD).

• Functional Assay: ab133101 PPAR gamma Transcription Factor Assay Kit

  Different volumes of positive control with inhibitor (duplicates, +/-SD).

• Functional Assay: ab133101 PPAR gamma Transcription Factor Assay Kit

  Different volumes of positive control (duplicates, +/-SD).

• Functional Studies - PPAR gamma Transcription Factor Assay Kit (ab133101)

  Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133101.

  Panel B: PPAR gamma DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab133101 has been referenced in 21 publications.

• Ning Z et al. USP22 regulates lipidome accumulation by stabilizing PPAR? in hepatocellular carcinoma. Nat Commun 13:2187 (2022). PubMed: 35449157
• Comas F et al. Activation of Endogenous H2S Biosynthesis or Supplementation with Exogenous H2S Enhances Adipose Tissue Adipogenesis and Preserves Adipocyte Physiology in Humans. Antioxid Redox Signal 35:319-340 (2021). PubMed: 33554726
• Wagner G et al. LMO3 reprograms visceral adipocyte metabolism during obesity. J Mol Med (Berl) 99:1151-1171 (2021). PubMed: 34018016
• Xu C et al. Pharmacological Activation of RXR-α Promotes Hematoma Absorption via a PPAR-γ-dependent Pathway After Intracerebral Hemorrhage. Neurosci Bull 37:1412-1426 (2021). PubMed: 34142331
• Jiang Y et al. FGF21 Protects against Aggravated Blood-Brain Barrier Disruption after Ischemic Focal Stroke in Diabetic db/db Male Mice via Cerebrovascular PPAR? Activation. Int J Mol Sci 21:N/A (2020). PubMed: 32012810

View all Publications for this product